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. 2015 Mar 4;11(3):e1004986. doi: 10.1371/journal.pgen.1004986

Fig 7. High levels of RNAPII but low levels of transcripts are found at central domain.

Fig 7

(A) ChIP of RNA polymerase II (RNAPII) at endogenous cc2 and at the pMcc2 plasmid in wild-type cells (n = 3). rDNA: negative control. (B) qRT-PCR performed on total RNA extracted from exosome mutant cells (dis3–54) lacking endogenous cc2 and transformed with the pMcc2 plasmid (n = 3). Transcript levels are shown relative to act1 + (n = 3). (C) 5’RACE-PCR was performed on poly(A) purified RNA extracted from exosome mutant (dis3–54) cells lacking endogenous cc2 and transformed with the p3xLM or p3xOP plasmid. In the figure, TSSs are represented as bent arrows. (D) Analysis of promoter activity: ∼200 bp fragments from cc2 (region-L1,-L2,-M1,-M2,-O1,-O2,-P1,-P2) were placed upstream of a LacZ reporter gene. The levels of LacZ expression was assessed by measuring absorbance at 420 nm of cell lysates incubated with 2-Nitrophenyl-β-D-galactopyranoside (ONPG). nmt81: positive control with nmt81 promoter (n = 3).