Fig. 5.

K⁺- and L-glutamate-induced mobilization of Ca²⁺ in neuron-like cells derived from canine BMSCs after incubation with (closed circles) and without (open circles) bFGF (100 ng/ml). The cells were stimulated with either 50 mM KCl (A) or 100 µM L-glutamate (B). Images of Ca²⁺ response to KCl or L-glutamate in the fluorescent dye-loaded cells treated with bFGF were displayed in the upper panel. Green fluorescence shows the changes in intracellular Ca²⁺ concentration, indicating neuronal activation. Changes in intracellular Ca²⁺ concentration are displayed in the bottom panel. The scale bar is 200 µm.