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. 2015 Jan 7;11:2. doi: 10.1186/1744-8069-11-2

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© Nasirinezhad et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic of construct encoding recombinant peptides. The schematic shows the features of the construct. The CMV promoter from lentiviral vector pRRL (pRRLsinPPT.CMV.EM1/EM2/SHG.Wpre) is followed by the signal sequence preproNGF-β (BamHI-BglII) and the cDNAs encoding for SHG or EM1 or EM2 (BglII-XbaI). The locations of forward and reverse gene specific primers used to assess gene expression are shown by filled and open arrows respectively.