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. 2015 Jan 7;11:2. doi: 10.1186/1744-8069-11-2

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© Nasirinezhad et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunodot-blot analysis of CSF and spinal cord of treated rats. Immunodot-blot assay of CSF from the GFP control SCI animals and SCI animals injected by a mixture of viral vectors encoding SHG and EMs. No reactivity was seen in the CSF of GFP control SCI animals. The amount of each peptide spotted is indicated in the column on the left side of each panel. A: reactivity of CSF with endomorphin 1 and 2 antibody, B: reactivity of CSF with SHG antibody. C: detection of SHG in the spinal cord homogenate from EMs + SHG treated animals.