Figure 1.

Purification of recombinant GAS1 from insect cells. A) Ni-affinity chromatogram for GAS1 purification B) Western blot of fractions from the Ni-affinity chromatography peak at ca. 28 ml. Fractions of 1 ml were collected, and fractions from peak area at 23, 27, 29 and 33 ml were tested in the western blot. C) SDS-PAGE analysis of GAS1 purification (left lane, molecular weight marker, with sizes indicated; right lane, purified GAS1 after gel filtration; the gel was Coomassie stained.).