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. 2015 Feb 28;16:8. doi: 10.1186/s12858-015-0037-6

Table 1.

SAXS-derived size parameters for GAS1

I(0) (Guinier) 25.08
I(0) (Porod) 24.8
Dmax (nm) 10.5
Rg (Guinier/nm) 3.01
Rg (Porod/nm) 3.00
Porod volume (Vp) 54.2
Mw(theoretical) 29 158.3 g/mol
Mw(calc) (Guinier) 25.1 kDa
Mw(calc) (Porod vol.) 31.9 kDa (Vp/1.7)

The Guinier I(0)-value was calculated against an absolute reference (scattering of water relative to sample) [23] and the I(0) for the sample is then equal to the molecular weight. Molecular weight from the Porod volume is estimated according to Petoukhov et al. [24].