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. 2015 Feb 25;16(1):126. doi: 10.1186/s12864-015-1319-5

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© Hou et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Generation of recombinant Sjda proteins. A. Detection of purified GST-tagged recombinant Sjda protein. The molecular weight of Sjda-GST is 69 kDa. B. Detection of purified His-tagged recombinant Sjda protein. The molecular weight of Sjda-His is 43.5 kDa. Lane M, protein molecular weight markers; Lane 1, purified proteins were analysed by SDS-PAGE and stained with Coomassie brilliant blue; Lane 2, Western blotting of the recombinant protein with specific mouse anti-Sjda antibodies; and Lane 3, Western blotting of the recombinant protein with anti-GST-tag mAb (A) or anti-His-tag mAb (B).