Figure 1.

Sequence alignment of hCaM, cTnC and chimeras. Sequence alignment was performed with ClustalW. Residues are colored coded in terms of amino acid properties where green are hydrophobic, blue are polar/uncharged, black are positively charged, red are negatively charged and brown are aromatic. The four, 12-residue EF-hand Ca²⁺ binding loops are depicted in bold and underlined. The corresponding sequences from cTnC added to create the individual chimeras are outlined with boxes.