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. 2015 Feb 15;16:6. doi: 10.1186/s12858-015-0036-7

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© Jensen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The lifetime measurement of CaM-bound ANS. A solution containing 5 μM CaM and 50 μM ANS was excited with 345 nm from a NanoLED light source and its dynamic fluorescence decay (black) was monitored with TCSPC using a 200-ns window for 4000 channels. The prompt is shown in blue color. The decay was best fitted with a three-exponential decay (green line in upper panel) and the weighted residues are indicated in the lower panel. The calculated χ² is 0.986. The values from the fitting are shown in Table 2.