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. 2015 Feb 15;16:6. doi: 10.1186/s12858-015-0036-7

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© Jensen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Kinetics of Orai-CMBD to CaM and chimeras. A solution of 5 μM dansyl-CaM, dansyl-CaM(1TnC), or dansyl-CaM(3TnC) and 15 μM Orai-CMBD was quickly mixed with a solution containing 75 μM CaM in the presence of 2 mM Ca²⁺ and the fluorescence decay was monitored for 2 s for CaM (A) and CaM(1TnC) (B) and 5 s for CaM(3TnC) (C) (left panel). The decay was fitted with a single exponential decay and the resulting k _off values are shown in Table 4. The peptide dissociated from dansyl-CaM or chimeras from the mixing resulted in the intensity decrease as observed in the complexed and uncomplexed fluorescently labeled CaM or chimeras (right panel).