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. 2015 Feb 15;16:6. doi: 10.1186/s12858-015-0036-7

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© Jensen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Circular dichroism spectra of uncomplexed and complexed CaM. The CD spectra of CaM were recorded in the presence of Ca²⁺ (solid line) and Ca²⁺ and Orai-CMBD (dotted line). Their differential spectrum (dashed line) presents the structure of Orai-CMBD in the complex assuming no CaM structural change upon ligand binding. Note that the CD unit was intentionally shown in degrees of light rotation because the CD spectra of CaM and the complexed Orai-CBMD have a very similar mean residual ellipticity.