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. 2015 Feb 15;16:6. doi: 10.1186/s12858-015-0036-7

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© Jensen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Stern -Volmer plot of Trp76 fluorescence of Orai- CMBD quenched by acrylamide. A representative figure of Stern-Volmer quenching by acrylamide is shown for Orai-CMBD and CaM/Orai-CMBD based on steady state fluorescence. To ensure no free Orai-CMBD peptide in the complex, CaM or the chimeras were added into the solution of 5 μM Orai-CMBD until no significant fluorescence change was observed. The values from the fitting are shown in Table 3. Insert: the quenching was monitored by two lifetime components of Trp76 of Orai-CMBD in the complex.