Table 3.
Trp76 of Orai-CMBD fluorescence and Stern-Volmer quenching constants
| Protein | λ max (nm) | Trp fluorescence enhancement upon protein binding (fold) | K sv (M −1 ) acrylamide | K sv (M −1 ) KI |
|---|---|---|---|---|
| Orai-CMBD | 350 | n.a.a | 13.5 ± 0.4 | 13.1 ± 0.5 |
| CaM/Orai-CMBD | 335 | 1.82 ± 0.06 | 1.35 ± 0.08 | 1.31 ± 0.08 |
| (CaM)1TnC/Orai-CMBD | 335 | 1.61 ± 0.05 | 1.80 ± 0.09 | 1.75 ± 0.06 |
| (CaM)2TnC/Orai-CMBD | 336 | 1.44 ± 0.11 | 1.95 ± 0.10 | 1.85 ± 0.05 |
| (CaM)3TnC/Orai-CMBD | 336 | 1.75 ± 0.08 | 1.32 ± 0.05 | 1.21 ± 0.09 |
| (CaM)4TnC/Orai-CMBD | 337 | 1.76 ± 0.07 | 1.51 ± 0.04 | 1.22 ± 0.10 |
aNot applicable.