Table 4.
Thermodynamics and kinetics of Orai-CMBD to CaM and chimeras
| Protein | K a1 (M −1 ) | ΔH 1 (kcal/ mol) | K a2 (M −1 ) | ΔH 2 (kcal/ mol) | Binding model or N for one set of sites | k off (s −1 ) |
|---|---|---|---|---|---|---|
| CaM | 8.92 ± 1.03 × 105 | - 5.02 ± 0.13 | n.a.a | n.a. | N = 2.05 ± 0.30 | 1.41 ± 0.08 |
| CaM(1TnC) | 7.57 ± 1.10 × 105 | - 4.99 ± 0.14 | 1.70 ± 0.30 × 104 | - 3.33 ± 0.30 | Sequential | 2.44 ± 0.21 |
| CaM(2TnC) | 6.66 ± 0.20 × 105 | - 5.21 ± 0.21 | 1.39 ± 0.35 × 104 | - 3.79 ± 0.39 | Sequential | 2.64 ± 0.32 |
| CaM(3TnC) | 2.90 ± 0.12 × 105 | - 6.58 ± 0.05 | n.a. | n.a. | N = 1.93 ± 0.24 | 0.68 ± 0.25 |
| CaM(4TnC) | 5.77 ± 1.35 × 105 | - 5.34 ± 0.40 | n.a. | n.a. | N = 1.87 ± 0.15 | 0.72 ± 0.31 |
aNot applicable.