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. 2015 Mar 4;10(3):e0119338. doi: 10.1371/journal.pone.0119338

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© 2015 Fang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Northern blotting analysis of UNC50 mRNA expression levels in 12 paired HCC tissues (T) and adjacent non-cancerous tissues (N); 28S and 18S bands were used as the references. (B) Real-time PCR analysis of UNC50 expression levels in 44 paired tissues. Relative mRNA expression levels are normalized to β-actin; log2-transformed fold changes of HCC tissues compared to the adjacent non-cancerous tissues were calculated by the comparative cycle threshold (ΔΔCt) method. The paired tissues are ordered from low to high ΔΔCt values. Cutoff values were set to ±1 to identify the significance of changes. (C) Western blotting analysis of UNC50 levels in another 12 paired HCC tissues; β-actin was used as the reference.