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. 2015 Mar 4;10(3):e0119338. doi: 10.1371/journal.pone.0119338

Fig 3. Western blotting confirmation that UNC50 affects cellular EGFR pathway activity.

Fig 3

Cells (105 from each cell type) were seeded to 24-well plates in DMEM with 10% FBS (Complete medium). After adherence, cells were pre-treated with serum free DMEM for 24 hours before being stimulated with either flesh serum free DMEM, complete medium, or complete medium with 1ng/ml EGF for 8 hours. Lysates were then collected and analyzed for pEGFR, EGFR, β-actin, cyclin D1 and UNC50 by western blot. SF = serum-free DMEM.