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. 2015 Mar 4;10(3):e0119338. doi: 10.1371/journal.pone.0119338

Fig 4. UNC50 prompts EGF-induced cell cycle entry and proliferation.

Fig 4

(A) Flow cytometry analysis of cells stimulated with complete medium (10% FBS), complete medium with 1 ng/ml EGF (EGF) added for another 24 hours, or 10 μmol/ml erlotinib (Erlotinib) after EGF treatment. (B) MTT evaluation of cell proliferation status. Top: Cells were cultured in complete medium without change of medium; cell vitality was analyzed every 24 hours. Bottom: Cells were cultured in complete medium with 1 ng/ml EGF and replaced with fresh medium every 12 hours to sustain EGF levels. Cell vitality was analyzed every 24 hours. *: p<0.05, **: p<0.01.