Table 1. Characteristics and plasmid profiles of selected CTX-M-15-Kp (Kp) and CTX-M-15-producing E. coli (Ec) isolates.
| Subject | Months colonized | Isolate | PFGE-type a | Co-resistance b | Inc type c | Plasmid size (kb) d | Southern blot-hybridization | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Source | ID | bla CTX-M | IncFIIK | IncR | ||||||
| Mother child 1 | Index | BM | Kp2177 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 | |
| Child 1 | 18 | First | F | Kp2158 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 |
| Last | F | Kp734 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 | ||
| Child 2 | 19 | First | F | Kp2180 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 |
| Last | F | Kp723 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 | ||
| Child 3 | 22 | First | F | Kp2223 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 |
| Last | F | Kp798 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 | ||
| Child 4 | 24 | First | F | Kp2166 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | ~180 | ~70 |
| Last | F | Kp848 | 1 | GEN | FIIK | ~180 | ~180 | ~180 | - | ||
| Child 5 | 9 | First | F | Kp2189 | 1 | GEN, STX | FIIK, R | ~70, ~180 | ~180 | nd | nd |
| F | Ec567 | nd | - | I1, FIA | ~10, ~20, ~40, ~75 | ~75 | nd | nd | |||
(BM) Breast milk; (F) Faeces; (GEN) gentamicin; (SXT) trimethoprim/sulfamethoxazole; (nd) not determined
aPFGE of XbaI-digested DNA.
bGradient test (Liofilchem).
cPCR-based replicon typing.
dPFGE of S1-nuclease digested DNA.