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. 2015 Feb 12;12:21. doi: 10.1186/s12985-015-0252-1

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© Lehmann et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Supernatants from MH-S cells increase chemotaxis of murine promyelocyte MPRO cells in a CCR1 dependent manner. MPRO cells were tested for chemotaxis towards control medium (CM), human CCL3 (20 ng/ml) and supernatants from MH-S cells (16 h) challenged with LPS (1 μg/ml) or infected with MVA or VACV WR at an MOI as indicated using a 96-well Multi-Screen-MIC plate with a 5 μm pore-sized filter (Millipore). 75.000 cells were placed in the upper part and cells migrated into the lower part were counted after 3 h. Where indicated MPRO cells were pre-incubated with 20 nM of the CCR1 antagonist J 113863 for 5 min prior to running the assay. Data are means ± SD (n = 3).