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. 2015 Feb 21;15:62. doi: 10.1186/s12870-015-0448-y

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© Tan et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TEM and measurement of SCW thickening for control and T ₂ transgenic plants. (A) Xylem cell wall of WT, (B) Xylem cell wall of T₂ dwarf 35S:HvCesA4, (C) Sclerenchyma cell wall of WT and (D) Sclerenchyma cell wall of T₂ dwarf 35S:HvCesA4. Scale bar is 1 μM for (A, B) and 10 μM for (C, D). Cy = Cytoplasm of bundle sheath cell, ML = middle lamella. (E, F) Percent AIR extracted (w/w) from 35S:HvCesA4, 35S:HvCesA8 and control from stem tissues. (G) % reduction of xylem cell wall thickness as measured using ImageJ.