Table 1.
Transgenic T 1 35S:HvCesA4 | Transgene/Endogenous ratio | Endogenous HvCesA4 | Endogenous HvCesA7 | Endogenous HvCesA8 |
dwarf | 82% | 25% | 36% | 32% |
normal | 215% | 115% | 95% | 112% |
Transgenic T 1 35S:HvCesA8 | Transgene/Endogenous ratio | Endogenous HvCesA4 | Endogenous HvCesA7 | Endogenous HvCesA8 |
stunted | 79% | 6% | 7% | 4% |
brittle node | 644% | 44% | 42% | 14% |
normal | 518% | 32% | 35% | 44% |
Values are calculated as [(T/E)*100] to determine the ratio of transgene transcript levels to its corresponding endogenous gene expressed in control plants, where T = Average gene levels in transgenic plants and E = Average corresponding endogenous gene level in control plants. For endogenous genes, percentage expression in transgenic cf. control plants were calculated.