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. 2015 Mar 4;9(3):e0003544. doi: 10.1371/journal.pntd.0003544

Fig 6. siRNA dose-dependent knockdown of Spermatogenesis-associated factor, Enolase phosphatase e-1 and Chaperonin-60kD.

Fig 6

Scrambled siRNAs for (A) Spermatogenesis-associated factor, (C) Enolase phosphatase e-1 and (E) Chaperonin-60kD were subjected to transfection across a range of concentrations (0–50 nM) and then infected with CHIKV. No virus inhibition was observed for any scrambled siRNAs when compared to transfection control (TC). Gene-specific siRNAs against (B) Spermatogenesis-associated factor, (D) Enolase phosphatase e-1 and (F) Chaperonin-60kD were transfected into C6/36 cells at different concentrations (0–50 nM) and subjected to CHIKV infection. Significant dose-dependent inhibition of CHIKV infection was observed in spermatogenesis-associated factor from 30 nM to 50 nM, with approximately 2-log unit PFU/ml reductions. Significant dose-dependent increases in CHIKV titers were observed with enolase phosphatase e-1 and chaperonin-60kD from 10 nM to 50 nM, with approximately 2-log unit PFU/ml increases. Cell viability upon drug treatment is represented by the line graphs. The asterisk indicates *p values <0.05, **p values of <0.01 and ***p values <0.0001 by Student’s t test. Asterisks indicate statistically significant results relative to control group (■).