Fig 2. Wild type RpsL restricts the replication of L. pneumophila strains in macrophages.
A. Strain Philadelphia 1 was unable to replicate in bone marrow-derived macrophages. B. Conversion of the rpsL K88R allele of the laboratory strain Lp02rpsLK88R into wild type abolished its ability to grow in macrophages. Experiments were performed in macrophages from A/J mice as described in Fig 1. With the exception of strains Lp02rpsLK88R and Lp03, which harbor a spontaneous K88R mutation in rpsL, the rpsL locus of all other bacterial strains was introduced by genetic manipulation. Infections were performed in triplicate and data shown were from one representative of three experiments with similar results.