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. 2015 Mar 4;10(3):e0118284. doi: 10.1371/journal.pone.0118284

Fig 3. Transient transfection of BMECs with miR-486 mimics/inhibitor, led to changes in miR-486 and PTEN.

Fig 3

A: qRT-PCR analysis of miR-486 expression in BMECs after transfection with miR-486 mimics or negative control (miR-NC). B: qRT-PCR analysis of miR-486 expression in BMECs after treatment with miR-486 inhibitors or the negative control (Anti-NC). C: qRT-PCR analysis of PTEN expression in BMECs after treatment with miR-486 mimics or negative control (miR-NC). D: qRT-PCR analysis of PTEN expression in BMECs after treatment with miR-486 inhibitors or negative control (Anti-NC). E: Western blotting analysis of PTEN protein expression in BMECs after treatment with miR-486 mimics or negative control (miR-NC). F: Western blotting analysis of PTEN protein expression in BMECs after treatment with miR-486 inhibitors or negative control (Anti-NC). All of the above experiments were performed in triplicate. G: Confocal microscopy analysis of PTEN expression in BMECs after treatment with miR-486 mimics (30 μM), negative control (miR-NC) (30 μM), miR-486 inhibitors (30 μM), or negative control (Anti-NC) (20 μM). (a): PTEN, (b): nuclear staining with propidium iodide, (c): merged images of (a) and (b). MiR-486 targets PTEN and represses the expression of PTEN mRNA and protein. The indicated values are the means±SD, ***P<0.0001.