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| Gene | Primer Sequence (5′–3′) | Amplicon size |
|---|---|---|
| PTEN | F:CACCTATTCCTCAGCCCTTAT | 273bp |
| R:AACCCTCATTCAGACCTTCAC | ||
| mTOR | F:ATGCTGTCCCTGGTCCTTATG | 178bp |
| R:GGGTCAGAGAGTGGCCTTCAA | ||
| AKT | F:TAAAGAAGGAGGTCATCGTGG | 181bp |
| R:CGGGACAGGTGGAAGAAAA | ||
| β-actin | F:AAGGACCTCTACGCCAACACG | 249bp |
| R:TTTGCGGTGGACGATGGAG |
