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Funtional analysis of TCF4M overexpression in EC109 cells. TCF4M construct and TOPflash reporter were co-transfected into EC109 cells, combined with or without β-catenin expression vector. The transcription activity was determined by relative luciferase activity (A). Total RNA was isolated for realtime PCR to detect the gene expression of CyclinD1 and MMP7 (B). N: empty vector.
