Table 2. Throughput and quality control of RNA-Seq data from Gaocheng and Satsuma mandarin libraries.
Sample a | Clean bases | Q20(%) b | Total mapped(%) c | Uniquely mapped(%) | Splice reads(%) d |
---|---|---|---|---|---|
GCS1 | 2.38G | 98.53 | 84.98 | 83.28 | 28.10 |
GCS3 | 2.61G | 97.22 | 82.09 | 79.02 | 27.62 |
GCS6 | 2.27G | 98.52 | 85.42 | 82.75 | 29.01 |
SMS1 | 2.80G | 98.60 | 88.00 | 85.64 | 27.44 |
SMS3 | 2.61G | 98.58 | 88.60 | 86.28 | 28.67 |
SMS6 | 2.72G | 98.54 | 88.65 | 87.30 | 29.28 |
a GCS1. GCS3 and GCS6 represent stages S1, S3 and S6 of Gaocheng, while SMS1, SMS3 and SMS6 represent S1, S3 and S6 of Satsuma mandarin.
b The percentage of sequences with sequencing error rate lower than 1%.
c The percentage of clean bases mapped to the reference genome.
d The percentage of sequences aligned to two transcripts.