Figure 5. Inhibition of HIV infection and viral dissemination by candidate microbicides.

Penile tissue explants were exposed to compound (PMPA (Figure 5a), PRO 2000 (Figure 5b) and Cyanovirin-N (Figure 5c)) just prior to exposure to HIV-1BaL in the presence of compound for 2 hours. Virus and compound were removed and explants cultured overnight. Explants were then separated from any cells that had migrated from the tissue and cultured separately for 10 days (black bars). Viral replication was determined by measurement of p24 release into culture supernatant and is expressed as % of the untreated control. Viral dissemination by migratory cells (white bars) was determined by p24 release following 4 day co-culture with indicator T cells. Data represent the mean ± standard error of 3 separate donors, where each donor was tested in triplicate.