Fig. 1.

Isolation of tumor cells from the PCa-118b tumor. A, x-ray of PCa-118b tumors in SCID mice detected the presence of bone within the PCa-118b tumors. The location of the tumors is outlined by circles. B, Procedure for the isolation of PCa-118b tumor cells from the tumor. The PCa-118b tumor cells in culture exhibit cuboidal epithelial morphology. C, FACS using antibodies against human EpCAM and mouse H2K indicated the enrichment of the epithelial cell marker in the isolated tumor cell preparation. D, qRT-PCR for the relative expression of human versus mouse GAPDH in the PCa-118b tumor versus the purified PCa-118b tumor cell preparation. E, SDS-PAGE analysis of conditioned medium from the isolated PCa-118 cells. Right panel, gel slices that were used in mass spectrometry analysis.