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. 2014 Dec 19;14(3):471–483. doi: 10.1074/mcp.M114.039909

Fig. 4.

Fig. 4.

FGF3 and FGF19 function as both autocrine and paracrine factors in PCa-118b tumor. A, qRT-PCR for the expression of human FGF family genes detected FGF3, 9, and 19 messages in isolated PCa-118b cells. B, qRT-PCR for the expression of FGF receptors in PCa-118b cells showed that PCa-118b cells mainly expressed FGFR1. C, qRT-PCR using mouse-specific primers for the expression of FGF receptors in 2H11 endothelial cells showed that 2H11 cells expressed FGFR1. D, qRT-PCR using mouse-specific primers detected FGFR1 and FGFR2 in mouse osteoblasts. E, qRT-PCR for the expression of human KL and KLB in PCa-118b cells, mouse KL and KLB in 2H11 endothelial cells, and mouse KL and KLB in PMO. F, Effects of FGF3 and FGF19 on p44/42 and p38 phosphorylation. G, Stimulation of osteoblast proliferation by FGF3 and FGF19, as measured by increases in cell numbers over 3 days. *, p < 0.05. H, Effects of FGF2, FGF3, and FGF19 on osteoblast differentiation, as measured by alkaline phosphatase activity over 3 days. *, p < 0.05. I, FGF3 and FGF19 function as both autocrine and paracrine factors based on receptor expression and their effects on osteoblasts.