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. 2015 Jan 13;14(3):686–694. doi: 10.1074/mcp.M114.042697

Fig. 5.

Fig. 5.

The mRNA and protein expression level of several HSFs and HSPs is reduced in ann1–2/ann2 double mutant. A, Semi-quantitative RT-PCR analysis of the mRNA levels of HSF-A7A, HSF-A2, HSF-B1, and HSF-B2A in one-week-old wild type (Col-0) and ann1/ann2 double mutant (ann1/2) seedlings that were HS treated for the indicated time. The RT-PCR products of UBC are shown as the equal-loading control. B, Immunoblot analysis of the protein levels of HSP 17.7, HSP 25.3, HSP 70, and HSP 90 in wild- type and ann1–2/ann2 double mutant. One-week-old seedlings were treated with or without HS for the indicated time, total proteins were separated by SDS-PAGE, blotted onto nitrocellulose membrane and blotted with different HSP specific antibodies. Rubisco large subunit stained by Ponceau S is shown as the equal-loading control.