Table I. Early heat-regulated microsomal proteins identified by mass spectrometry. The heat regulated abundance changes were observed from at least five biological repeats. For MS/MS identification, number of unique peptides, sequence coverage of the identified proteins and the best matched scores are listed.

Spots	Accession number	Protein name	Unique peptides	Coverage (%)	Abundance change by HS	Score
Hormone biosynthesis
2586	At2g46370	Jasmonate-amido synthetase	3	6.26	up	30.19
3984	At1g62380	ACC oxidase 2	8	32.81	up	70.28
4095	At3g44310	Nitrilase 1	9	37.28	down	100.28
4125	At3g44310	Nitrilase 1	12	50	down	130.29
4285	At3g44310	Nitrilase 1	6	25.72	down	70.27
Protein translation
3384	At1g57720	Translation elongation factor EF1B	5	12.35	up	50.23
3394	At1g57720	Translation elongation factor EF1B	4	15.5	down	50.24
3421	At1g57720	Translation elongation factor EF1B	10	26.63	up	170.29
3415	At3g13920	Eukaryotic translation initiation factor 4A-1	7	28.16	up	100.31
3424	At3g13920	Eukaryotic translation initiation factor 4A-1	8	34.22	down	120.32
3804	At4g20360	GTP binding Elongation factor Tu family protein	9	27.73	up	80.31
Calcium signaling
4181	At1g35720	Annexin 1	2	6.308	up	40.24
4191	At1g35720	Annexin 1	5	15.77	up	70.28
Protein folding
2371	At5g42020	Luminal binding protein (BIP2)	3	4.8	up	30.19
2407	At5g42020	Luminal binding protein (BIP2)	6	11.98	up	50.24
6119	At3g62030	Cyclophilin 20–3 (CYP20–3)	5	24.52	up	50.25
Metabolism
1969	At1g08520	CHLD subunit of the Mg-chelatase enzyme	16	25.58	up	160.23
3874	At1g73110	Nucleoside triphosphate hydrolase	14	40.05	up	170.27
4097	At2g05830	5-methylthioribose-1-phosphate isomerase	7	25.67	up	80.24
4113	At5g15650	AtRGP2	4	11.67	up	40.23
4809	At2g34470	Urease accessory protein G (UREG)	5	26.55	up	50.31
5092	At4g30530	Gamma-glutamyl peptidase	3	17.60	up	30.26
5128	At3g02780	Isopentenyl diphosphate isomerase 2	5	19.01	up	50.28
5116	At3g11930	Adenine nucleotide alpha hydrolases like	2	11.5	up	20.19
Others
2060	At1g33790	Jacalin lectin family protein	4	11.69	up	40.17
2062	At1g33790	Jacalin lectin family protein	14	36.85	up	120.31
4734	At4g39090	Cysteine proteinases	3	9.24	down	30.22