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Purification of phospho-Lyn SH2 domain. A Flow chart summarizing the steps carried out in purification of Lyn pSH2. B Western blots showing the enrichment of phospho-SH2 by IMAC. FT denotes flow-through. C Phospho- and unphospho-SH2 were separated by cation exchange column. Top Absorbance chromatograph (280 nm) showing three peaks. Bottom The SRM-MS ion currents associated with pY194- or Y194-containing peptide in three peaks. D Western blot analysis of purified SH2 and pSH2.
