Fig. 3.

Lyn pSH2 binds pY-peptides with decreased affinity compared with SH2. A Venn diagram for pY sites that bound to Lyn SH2, pSH2, and identified by pY immunoprecipitation from Mv4–11 cell lysate derived peptides. B Sequence logos based on the 69 pY sites that bound to SH2 (top), 18 pY sites that bound to pSH2 (middle), and 531 total pY sites identified in Mv4–11 cells (bottom). C Heat map based on quantitative binding profiles of pY sites to SH2 and pSH2, sorted by fold-change in descending order. The gene name, pY site, surrounding sequences, and fold-change are shown. D Dissociation constants of Lyn SH2 or pSH2 and pY peptides, as indicated.