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. 2015 Feb 15;5(5):489–503. doi: 10.7150/thno.10069

Figure 4.

Figure 4

Fluorescence imaging of liposomes. In vitro (A, B) and in vivo (C) fluorescence images of liposomes loaded with nanoparticles and Rhodamine-100 as acquired with an IVIS-Lumina camera. A: Visible picture of the 96 well plate used as phantom, showing the columns of wells loaded with decreasing concentrations (top to bottom) of empty liposomes (white triangle), liposomes loaded with Rhodamine-100 (green triangle) and liposomes loaded with Rhodamine-100 and Nanotex (blue triangle). B: Fluorescence image of the same phantom using the Green Fluorescent Protein (GFP) filter. Note the increased fluorescence of the liposomes containing Rhodamine-100 only as compared to those containing Rhodamine-100 and Nanotex. C: In vivo images of a mouse injected with liposomes containing Rhodamine-100 and Nanotex (left side arrow), or liposomes containing Rhodamine-100 only (right side arrow).