Table 1.

Assignments of phospholipid and ω-3 PUFA-EE ¹H NMR resonances in water and chloroform suspensions.

	Resonance number	Assignment a)	δHDO b)	δCDCl3 c)
Liposomes	1	H16, H53	0.9	0.89-0.86 (m)
	2	H17-28, H38-41, H47-52	1.32	1.25 (m)
	3	H29, H37	n.o. d)	1.57 (m)
	4	H42-46	n.o. d)	2.02 (m)
	5	H30, H36	n.o. d)	2.34-2.26 (dt)
	6	H8, H10, H11	n.o. d)	3.28 (t)
	7	H6	n.o. d)	3.77 (t)
	W	Water	4.79	-
	8	H12	n.o. d)	4.05 (m)
	9	H14	n.o. d)	4.13 (m)
	10	H5	n.o. d)	4.37 (m)
	11	H15	n.o. d)	5.23 (m)
	12	H43, H45	n.o. d)	5.33 (m)
ω-3 PUFA-EE	13	H23	1.03	0.97 (t)
	14	H1	1.27	1.25 (t)
	15	H6	1.73	1.7 (q)
	16	H7, H22	2.19-2.14	2.32-2.07 (m)
	17	H5	2.32	2.3 (t)
	18	H10, H13, H16, H19	2.9-2.87	2.85-2.8 (m)
	19	H2	4.12	4.12 (t)
	W	Water	4.79	-
	20	H25-34	5.46-5.40	5.38-5.30 (m)

^a) Resonances 1-20 in Figure 2 are assigned to specific protons of the corresponding PtdCho and ω-3 PUFA-EE molecules (see Scheme 1 for proton numbering).

^b) Chemical shifts are measured in ppm and referenced to TSP at 0 ppm.

^c) Chemical shifts are measured in ppm and referenced to TMS at 0 ppm.

^d) The resonances marked with “n.o.” are not observable by high resolution ¹H HRMAS because of dynamical restrictions in the bilayer.