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. 2015 Feb 15;5(5):515–529. doi: 10.7150/thno.10319

Figure 5.

Figure 5

Epifluorescence photomicrographs of (A-C) B16F10 or (E-G) MC-38 tumour tissue stained for αV (green), β3 (red) and nuclei counterstain with DAPI (blue). (A,E) Showing αV expression with nuclei, (B,F) β3 expression with nuclei, and (C,G) merged 3 colour channels for visual co-localisation of αVβ3 expression (yellow). (D,H) Equivalent bright field micrographs in which c(RGDyK)-MPIO are clearly visible (arrowheads). (B,C,F,G,) c(RGDyK)-MPIO may also appear as red under this protocol (arrowheads). (I,J) Immunohistochemical micrographs from B16F10 (I) and MC38 (J) tumours showing vessels (CD31) stained brown; again bound c(RGDyK)-MPIO are clearly visible (arrowheads). (K,L) Quantitation of MPIO density per μm2 tumour area for both c(RGDyK)-MPIO and c(RADyK)-MPIO treated groups in the B16F10 (K) and MC38 (L) models. (M) Graph showing quantitation of total vessel area per tumour. Data are presented as Mean + S.E.M. in bar charts. Scale bars = 20 μm. *P < 0.05; **P < 0.005; ***P < 0.0001.