Table II. Identification of significantly altered proteins after AT treatment by Mass Fingerprint approach.
| # Master spot | Putative identification | Homology to protein (Genebank) | Protein score | Protein score C.I. % | Protein MW | Protein PI | Peptide count | Total ion score | Total ion C.I. % | T-test value | Fold change |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 105 | Elongation factor 2 | Salmo salar | 212 | 100 | 96406.4 | 6.19 | 21 | 76 | 99.995 | 0.0462 | −1.748 |
| gi 223647986 | |||||||||||
| 265 | 78kDa glucose/regulated protein Salmo salar | Salmo salar | 538 | 100 | 72539.3 | 5 | 28 | 288 | 100 | 0.0042 | 2.469 |
| gi 213511032 | |||||||||||
| 813 | GL16441 | Drosophila persimilis | 76 | 98.619 | 21738.9 | 10.33 | 10 | – | – | 0.0121 | −1.498 |
| gi 195164237 | |||||||||||
| 1052 | Phosphoglycerate kinase 1 | Salmo salar | 315 | 100 | 44917.1 | 8.31 | 11 | 257 | 100 | 0.0002 | −1.307 |
| gi 197631857 | |||||||||||
| 1539 | Branched chain amino acid aminotransferase | Zunongwangia profunda SM-A87 | 73 | 97.244 | 40105.2 | 5.63 | 11 | – | – | 0.0359 | 1.423 |
| gi 295134966 | |||||||||||
| 1782 | hypothetical protein | Salmo salar | 69 | 92.751 | 6919.7 | 9.4 | 4 | 29 | 0 | 0.0414 | −1.526 |
| gi 281416447 |
Mascot score: Probability Based Mowse Score: Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 83 are significant (p < 0.05). MALDI-TOF-TOF peak lists were searched against a NCBInr 10997816 protein database using MASCOT TM software with the following settings: Type of search: Sequence Query; Enzyme: Trypsin; Fixed modifications: Carbamidomethyl (C); Variable modifications: Oxidation (M); Mass values: MONOISOTOPIC; Protein Mass: Unrestricted; Peptide Mass Tolerance: ± 100 ppm; Fragment Mass Tolerance: ± 0.2 Da; Max Missed Cleavages: 1; Instrument type: MALDI-TOF-TOF.