Table III. Identification of significantly altered proteins after CBZ treatment by Mass Fingerprint approach.
| # Master spot | Putative identification | Homology to protein (Genebank) | Protein score | Protein score C.I. % | Protein MW | Protein PI | Peptide count | Total ion score | Total ion C.I. % | T-test value | Fold change |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 272 | Elongation factor 2 | Salmo salar | |||||||||
| gi 223647986 | 212 | 100 | 96406.4 | 6.19 | 21 | 76 | 99.995 | 0.0087 | −1.644 | ||
| 395 | Contactin 1a precursor | Danio rerio | |||||||||
| gi 136256388 | 70 | 94.749 | 115044.4 | 6.19 | 15 | – | – | 0.0168 | −1.576 | ||
| 404 | TenA family transcriptional activator | Marinomonas sp. MWYL1 | |||||||||
| gi 152997829 | 71 | 95.632 | 25638.9 | 5.16 | 9 | – | – | 0.0285 | −1.652 | ||
| 559 | Transketolase | Salmo salar | |||||||||
| gi 213511480 | 165 | 100 | 68896.6 | 6.18 | 20 | – | – | 0.0406 | 1.445 | ||
| 1020 | Phosphoglycerate kinase 1 | Salmo salar | |||||||||
| gi 197631857 | 315 | 100 | 44917.1 | 8.31 | 11 | 257 | 100 | 0.0272 | −1.754 | ||
| 1027 | Fumarylacetoacetase | Osmerus mordax | |||||||||
| gi 225706644 | 71 | 95.829 | 49875.9 | 6.27 | 5 | 53 | 99.485 | 0.0211 | 1.87 | ||
| 1052 | Phosphoglycerate kinase 1 | Salmo salar | |||||||||
| gi 197631857 | 315 | 100 | 44917.1 | 8.31 | 11 | 257 | 100 | 0.0272 | −1.754 | ||
| 1061 | unknown | Arabidopsis thaliana | |||||||||
| gi 116830485 | 69 | 92.582 | 8922.7 | 9.3 | 6 | – | – | 0.0281 | −1.206 | ||
| 1122 | Acetyl-CoA acetyltransferase, cytosolic | Salmo salar | |||||||||
| gi 213513638 | 243 | 100 | 41722 | 8.07 | 8 | 196 | 100 | 0.0057 | −1.279 | ||
| 1323 | Glyceraldehyde-3-phosphate dehydrogenase | Salmo salar | |||||||||
| gi 209737954 | 468 | 100 | 36097.4 | 7.12 | 21 | 238 | 100 | 0.0006 | 1.704 |
Mascot score: Probability Based Mowse Score: Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 83 are significant (p < 0.05). MALDI-TOF-TOF peak lists were searched against a NCBInr 10997816 protein database using MASCOT TM software with the following settings: Type of search: Sequence Query; Enzyme: Trypsin; Fixed modifications: Carbamidomethyl (C); Variable modifications: Oxidation (M); Mass values: MONOISOTOPIC; Protein Mass: Unrestricted; Peptide Mass Tolerance: ± 100 ppm; Fragment Mass Tolerance: ± 0.2 Da; Max Missed Cleavages: 1; Instrument type: MALDI-TOF-TOF.