Stable-isotope dilution (SID), phospholipid-depleted reverse polynomial dilution (RPD), and nondepleted RPD calibration curves for target peptides.
A, (AMBP) - HHGPTITAK peptide. Peak area ratio-to-heavy scaling factor for RPD techniques = 10. B, (SRGN) - NLPSDSQDLGQHGLEED peptide. C, (SPP24) - VNSQSLSPYLFR peptide. Peak area ratio-to-heavy scaling factor for RPD techniques = 2. D, (SPP24) - VSAQQVQGVHAR peptide. E, (SCG1) - ADQTVLTEDEK peptide. F) (GUC2A) - VTVQDGNFSFSLESVK peptide. Please note that where noted, peak area ratios have been scaled for graphical comparison between SID, phospholipid depleted RPD, and nondepleted RPD calibration methods. Please refer to supplemental Table S11 for the regression equations.