Table III. Peak Area Ratio-to-Heavy Values in Sample Matrices of Varying Content Concentration and Complexity.
| Peptide | No-Matrix |
0.001 μg E.coli background |
0.01 μg E.coli background |
0.1 μg E.coli background |
0.1 μg Phospholipid-depleted Plasma background |
0.1 μg Plasma background |
Per Peptide | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peak Area Ratio to Heavy | Precision (%CV) | Peak Area Ratio to Heavy | Precision (%CV) | Peak Area Ratio to Heavy | Precision (%CV) | Peak Area Ratio to Heavy | Precision (%CV) | Peak Area Ratio to Heavy | Precision (%CV) | Peak Area Ratio to Heavy | Precision (%CV) | p-Valueb | |
| HHGPTITAKPTITAK | 0.85 | 3.29 | 0.30 | 9.88 | 0.28 | 8.00 | 0.29 | 5.37 | 0.33 | 6.01 | 0.29 | 4.02 | <0.01 |
| NLPSDSQDLGQHGLEED | 1.43 | 3.65 | 0.91 | 5.55 | 0.99 | 2.19 | 0.89 | 9.85 | 0.95 | 10.11 | 0.96 | 7.97 | <0.01 |
| VNSQSLSPYLFR | 1.11 | 2.67 | 0.51 | 5.97 | 0.50 | 5.21 | 0.49 | 6.03 | 0.53 | 4.75 | 0.55 | 5.51 | <0.01 |
| VSAQQVQGVHAR | 1.80 | 5.70 | 1.31 | 11.45 | 1.56 | 7.98 | 1.38 | 2.31 | 1.50 | 9.80 | 1.53 | 8.88 | <0.01 |
| Matrix p-Valuea | – | – | <0.01 | 0.20 | 0.01 | 0.87 | <0.01 | 0.86 | <0.01 | 0.33 | <0.01 | 0.66 | |
a Matrix p value refers to the combined comparison across matrices for all peptides by a Repeated Measures ANOVA. The p values stated are the pairwise comparisons against No-Matrix. There were no significant differences between matrices otherwise.
b The Per-peptide p value refers to the comparison of the replicate peak area ratio-heavy values across matrices for the individual peptide. Peak area ratio-to-heavy values for all peptides were significantly different when a biological matrix was introduced. Italics denotes p values of significant difference.