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. 2014 Dec 8;8(1):e13783. doi: 10.5812/jjm.13783

Table 1. Primers Used in This Study.

Primers Sequence (5´ to 3´) PCR Cycles Gene and Its Size, bp
Denaturation Annealing Extension
VEB-1 F CGACTTCCATTTCCCGATGC 95°C, 1 min 60°C, 1 min 72°C, 1 min 30 blaVEB643
VEB-1 R GGACTCTGCAACAAATACGC
PER-1 F ATGAATGTCATTATAAAAGC 94°C, 30 s 51.5°C, 1 min 72°C, 1 min 25 blaPER925
PER-1 R AATTTGGGCTTAGGGCAGAA
TEM F GAGTATTCAACATTTCCGTGTC 94°C, 30 s 57°C, 1 min 72°C, 1 min 30 blaTEM861
TEM R TAATCAGTGAGGCACCTATCTC
SHV F AAGATCCACTATCGCCAGCAG 94°C, 30 s 64°C, 1 min 72°C, 1 min 25 blaSHV231
SHV R ATTCAGTTCCGTTTCCCAGCGG
CTX-m1 F GACGATGTCACTGGCTGAGC 94°C, 30 s 56°C, 1 min 72°C, 1 min 25 blaCTX-M1 499
CTX-m1 R AGCCGCCGACGCTAATACA