Table 1. Primers Used in This Study.
| Primers | Sequence (5´ to 3´) | PCR | Cycles | Gene and Its Size, bp | ||
|---|---|---|---|---|---|---|
| Denaturation | Annealing | Extension | ||||
| VEB-1 F | CGACTTCCATTTCCCGATGC | 95°C, 1 min | 60°C, 1 min | 72°C, 1 min | 30 | blaVEB643 |
| VEB-1 R | GGACTCTGCAACAAATACGC | |||||
| PER-1 F | ATGAATGTCATTATAAAAGC | 94°C, 30 s | 51.5°C, 1 min | 72°C, 1 min | 25 | blaPER925 |
| PER-1 R | AATTTGGGCTTAGGGCAGAA | |||||
| TEM F | GAGTATTCAACATTTCCGTGTC | 94°C, 30 s | 57°C, 1 min | 72°C, 1 min | 30 | blaTEM861 |
| TEM R | TAATCAGTGAGGCACCTATCTC | |||||
| SHV F | AAGATCCACTATCGCCAGCAG | 94°C, 30 s | 64°C, 1 min | 72°C, 1 min | 25 | blaSHV231 |
| SHV R | ATTCAGTTCCGTTTCCCAGCGG | |||||
| CTX-m1 F | GACGATGTCACTGGCTGAGC | 94°C, 30 s | 56°C, 1 min | 72°C, 1 min | 25 | blaCTX-M1 499 |
| CTX-m1 R | AGCCGCCGACGCTAATACA | |||||