Table 1. Gene-specific Primers Used in Polymerase Chain Reaction a.
Primer Sequence (5′ to 3′) | Length, nt | Accession Number |
---|---|---|
mazF | 288 | EG11249 |
(F) ATGGTAAGCCGATACGTACCC | ||
(R) TGGGGCAACTGTTCCTTT | ||
relE | 267 | EG11131 |
(F) GACGAGCGGGCACTAAAGGAAT | ||
(R) TCAGAGAATGCGTTTGACCG | ||
hipA | 1314 | EG10443 |
(F) CTTGTCACTTGGATGAACAACCAG | ||
(R) TCACTTACTACCGTATTCTCGGCT | ||
ccdB | 272 | P62554 |
(F) GAGAGAGCCGTTATCGTCTGTT | ||
(R) TCCCCAGAACATCAGGTTAATG | ||
MqsR | 194 | EG13023 |
(F) ACGCACACCACATACACGTT | ||
(R) GCCTGGGTCTGTAAACATCCT |
a in Toxin-antitoxin system.