Figure 1. Characterization of soc chs3-2D mutants.

(A) Morphology of soil-grown plants of the indicated genotypes. The photograph was taken when the plants were 21 days old. (B) Quantification of Hyaloperonospora arabidopsidis (H.a.) sporulation on the indicated genotypes following inoculation with pathogen isolate H.a. Noco2. Two-week-old soil-grown seedlings were sprayed with a spore suspension of H.a. Noco2 at a concentration of 100,000 spores/mL of water. The plants were then covered and incubated for seven days in a high humidity growth chamber. Spores were counted in water suspension using a hemocytometer (bars represent means of n replicates ± SD, n = 3 or 4 with 4 plants each). (C) PR1 and PR2 gene expression of the indicated genotypes as determined by RT-PCR. Both PR1 and ACTIN7 were amplified with 28 cycles while PR2 was amplified with 30 cycles. Two-week-old plate-grown seedlings were used for the analysis.