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. 2012 Feb 15;7(5):341–346. doi: 10.3969/j.issn.1673-5374.2012.05.003

Figure 2.

Figure 2

An amyloid precursor protein intracellular domain (AICD)-containing complex directly binds to the promoters of CaMKIIα and GluR-2 genes.

(A) Input-ChIP-DNA (1), negative control rat IgG (2) and AICD-ChIP-DNA (3) were amplified via PCR, using primer pairs for the promoter regions of five genes associated with learning and memory, and the products were resolved on 1.5% agarose gels followed by ethidium bromide staining.

(B) The two fragments, amplified from AICD-ChIP-DNA using primers against the promoter regions of the CaMKIIα and GluR-2 genes, were sequenced and are shown as partial sequences of the original color charts.

BDNF-PI: Brain-derived neurotrophic factor promoter I; CREB: cAMP-response element binding protein; PKMζ: protein kinase M zeta; CaMKIIα: alpha-calcium-calmodulin kinase II; GluR-2: glutamate receptor-2; ChIP: chromain immunoprecipitation.