Table 2.
Cytotoxic Effect of TdM Fractions on MDA-MB-231 Cells
| Fraction (eluent) | IC50 (μg/mL) |
|---|---|
| F1 (A, 60:40) | NA |
| F2 (A, 40:60) | 64.3±5.0 |
| F3 (A, 20:80) | 59.4±1.6 |
| F4 (B, 90:10) | 32.4±2.0 |
| F5 (B, 80:20) | 94.0±3.0 |
| F6 (B, 70:30) | NA |
| F7 (A, 60:40) | NA |
| F8 (A, 50:50) | NA |
| F9 (A, 30:70) | NA |
| F10 (B, 20:80) | NA |
The effect of the fractions of methanolic extract on tumor cells was examined by an MTT assay. The cells were treated with various concentrations for 72 h. Data from three independent experiments are expressed as the mean±SD.
TdM, methanolic extract of Turnera diffusa; NA, no activity; A, mixture hexane/CH2Cl2; B, mixture CH2Cl2/MeOH.