Fig. 2.—
Phylogenetic placement of C. rosea within Hypocreales. A phylogenomic analysis was performed with the program CVTree on whole predicted proteomes of the included species. Bootstrap support values ≥97% are associated with lineages. Branch lengths of the species topology generated by CVTree was calculated with PAML, based on a five-gene alignment including actin, calmodulin, glyceraldehyde 3-phosphate dehydrogenase, DNA-directed RNA polymerase II subunit B, and translation elongation factor 1 alpha. The species phylogeny was calibrated to the fossil record by setting the base of the Sordariomycetes to 335 Ma. The included species represents the order Hypocreales: C. rosea (family Bionectriaceae), F. graminearum, F. oxysporum f. sp. lycopersici, F. solani, F. verticillioides (family Nectriaceae), T. atroviride, T. reesei, T. virens (family Hypocreaceae), the order Sordariales (N. crassa), and the order Magnaporthales (M. oryzae). M, mycoparasite; P, plant pathogen; S, saprotroph.