Table 1.
Primer Pairs Used for Riboprobe Preparation and qRT-PCR Analysis
| Gene | ISH |
qRT-PCR |
||
|---|---|---|---|---|
| 5′–3′ | 5′–3′ | |||
| cry1a | F: | GAGAGCAGTTTCTTTTTTGG | F: | CAGGCGTGGAGGTGATAG |
| R: | AAGCCTCTGGGTTTTTATC | R: | TGGAAGCGCTTATACGTG | |
| cry1b | F: | GGATCTCCCACACACTCTATG | F: | ACACCGGTCAGTGATGATC |
| R: | CACGTGTGAGGAAGCAAG | R: | TGGACAGTCCCTCTGTTTC | |
| cry3a | F: | GGACTGACATAACGTTAAAAG | F: | GCTGTTGCATGTTTCCTC |
| R: | CAGTCTGCATCCAATAGAAG | R: | CAGTCTGCATCCAAATAGAAG | |
| cry3b | F: | TCTGCATTATTGACAGCTTG | F: | CCGGTGGAGAATCAGAAG |
| R: | CCCCACAGGACAGTAACAG | R: | TTCGGTCGCTCAAAGTTC | |
| cry2 | F: | ATTTCCTTGGAACTTTTACG | F: | GACATGCAGTAGCCTGTTTC |
| R: | CGCATCCAACAGCAACTC | R: | CGCATCCAACAGCAACTC | |
| cry4 | F: | CTACGCACAGTCGAAGAAC | F: | CGAACCTTCTACCACAGACTC |
| R: | CTTCTGGGTCGTAAAACATTC | R: | AGCGACGGTGTAGAAGAAC | |
| cry5/6-4phr | F: | CTGGGTGTGCAAGTTTGAG | F: | ACCCATTCCTGCTCCAAC |
| R: | ATGGATGGACTCGCTTTG | R: | CAGTCCAAGATCCTCAAGAG | |
| Rpl-13a | F: | GGACTGTAAGAGGTATGCTTC | ||
| R: | GATGCCATCAAACACCTTC | |||
| EF1α | F: | GAGGCCAGCTCAAACATG | ||
| R: | TCAAGGGCATCAAGAAGAG | |||
| prkcα | F: | GGACTCATACACCAAGGAATG | ||
| R: | GCTTGGCACATTCATCAC | |||