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Titration of immunized mouse serum by ELISA. Wells were coated with CD44v6 peptide, KLH-peptide, or BSA-peptide, and the immunized mouse serum was titrated in ELISA, using peroxidase conjugated sheep anti-mouse immunoglobulin as the secondary antibody. The mouse with higher serum antibody titer for anti-KLH-conjugated CD44v6 synthetic peptide was used for cell fusion.
