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. 2015 Mar 1;35(3):168–175. doi: 10.1089/jir.2013.0139

FIG. 4.

FIG. 4.

SDS-PAGE and western blotting analysis on purified rBoIFN-α1 and rBoIFNαA. (A) Purified rBoIFN-α1 and rBoIFNαA separated on 15% SDS-PAGE and stained with Coomassie Brilliant blue G-250. M, sizes (KD) of molecular-weight markers; Lane 1: supernatant of Pichia pastoris culture transformed with pPICZaA plasmid (control); Lane 2: supernatant of P. pastoris culture transformed with pPICαA-BoIFN-α1 plasmid number induced; Lane 3: rBoIFN-α1; Lane 4: rBoIFNαA. (B) Western blot analysis of rBoIFN-α1 and rBoIFNαA with a goat anti-rabbit IgG conjugated to horseradish peroxidase antibody against rabbit anti-bovine IFNα polyclonal antibody. M, sizes (KD) of molecular-weight markers; Lane 1: supernatant of P. pastoris culture transformed with pPICZaA plasmid (control); Lane 2: rBoIFN-α1; Lane 3: rBoIFNαA. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; rBoIFN-α1, recombinant mature BoIFN-α1.